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產品詳情
  • 產品名稱:IntegraseProtein

  • 產品型號:
  • 產品廠商:DIATHEVA
  • 產品文檔:
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簡單介紹:
IntegraseProtein
詳情介紹:
Purity >95?%
Background Integrase catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions. The enzyme activity takes place after virion entry into a cell and reverse transcription of the RNA genome in dsDNA. The full length HIV-1 integrase (288 amino acids) has three domains: the catalytic core, the C-terminal, and the N-terminal domains. Although all three domains are required for integration, it is thought that the catalytic core domain contains the active site responsible for catalysis of all the reactions of integration/disintegration. The C-terminal domain confers the capacity to bind both viral and host DNA. The structure and function of the N-terminal domain are presently unknown, but it contains a His2Cys2 zinc binding motif, suggesting a possible interaction with nucleic acid.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 20?mM phosphate buffer?pH 7.5, 1M NaCl, 10μM Zinc acetate, 1?mM DTT, 0.1?mM EDTA, 10?% (v/v) glycerol
Storage -20 °C
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